Applications for Imaging Workbench
Imaging Workbench 6 is an effective, practical and easy-to-use program
for ion and analyte concentration imaging. Imaging Workbench runs
under Windows 98, Windows 2000, and Windows XP. Imaging Workbench
is used for:
- Imaging of biological preparations in low light conditions.
- Control of experiments in which dual wavelength (ratiometric)
and single wavelength methods are used to determine ion concentration
information as a function of time.
- On-line analysis (i.e., during the experiment), with continuous
update of graphs and data export.
- Post-experimental analysis of imaging data.
- Synchronization of imaging with electrophysiology hardware and
Live Cell Imaging
Imaging Workbench is a powerful tool to monitor and measure fast
or slow timelapse events, including ion concentration changes, molecular
interactions, cell growth and differentiation, and other dynamic changes.
Fluorescence resonance energy transfer (FRET) is a distance-dependent
physical process by which energy is transferred nonradiatively from
an excited molecular fluorophore (the donor) to another fluorophore
(the acceptor) by means of intermolecular long-range dipole-dipole
coupling. Imaging Workbench can be used for FRET by customizing your
current imaging system.
FluoVis Imaging Systems with Imaging Workbench
Imaging Workbench is a key element of FluoVis, a customized turnkey
to determine ion concentration, dynamic fluorescence, intrinsic signal
and cell volume activity in live preparations using fluorescence and/or
bright-field microscopy. It also serves as a core for concurrent
imaging and electrophysiology or imaging
Concurrent Imaging and Electrophysiology
Imaging Workbench can be run on the same computer simultaneously
with pCLAMP 8 though 10 or with PatchMaster v2 or Pulse from Heka,
for concurrent imaging and electrophysiology. Image playback and analysis
allows simultaneous viewing of imaging and electrophysiology data
in the Graph window, sharing the same time axis and measurement cursors.
Concurrent Photometry and Electrophysiology
Imaging Workbench 6 can be built into a complete photometry solution
hardware from TILL Photonics (Germany) or Cairn Research (UK) and
software (pClamp) from
Axon Instruments/Molecular Devices. See Integrated
Imaging and Electrophysiology systems.